CGTase activity is inhibited by a high substrate concentration and by products formed during reaction,,,,. Therefore, this fact must be taken into account in order to improve the yield and selectivity for CD industrial production.

nitrogen source for CGTase production. Ca2+ influences the CGTase production and Zn2+ inhibits the enzyme. High CGTase activity was observed at 24 h of.

We discovered that CGTase has antimicrobial activity and induces resistance. In addition, we have revealed the key do-mains responsible for its hydrolytic activity and resistance induction. Further experiments demonstrated that CGTase is a potential func - Polyethylene glycol-6000 showed a 26 % increase in the CGTase activity. The kinetic parameters Km and Vmax were 10 mg/ml and 146 µmol/mg min, respectively, for purified CGTase. CGTase can catalyse four reactions: cyclization, coupling, disproportionation, and hydrolysis (Li et al., 2014b), though the hydrolysis activity is relatively weak (Costa et al., 2009).

Cgtase activity

β‐CGTase activity increased to 122.0 U/mL after optimization of glycerol, phosphate buffer, pH value, ammonium sulfate, temperature, methanol, and additives based on BSM, establishing a modified defined medium. 23 Aug 2017 When expression was induced at 25 °C for 32 h, and then the temperature was shifted to 30 °C, the extracellular α-CGTase activity at 90 h was 45 Catalytic activities[edit]. All of the CGTases can catalyze up to four reactions: cyclization, coupling, disproportionation and hydrolysis. All these activities share the 23 Sep 2016 megaterium in a submerged fermentation. Afterwards, the CGTase was purified partially and its activity to synthesize α-, β-, and γ-CDs was It was reported that CGTases are active on both starch fractions: amylose and amylopectin but the high amylopectin content were preferred for CGTase activity.

Activity and stability characteristics of an alkaline active cyclodextrin glycosyltransferase (CGTase) enzyme from the alkaliphilic Bacillus agaradhaerens LS-3C strain are reported. The enzyme displays unusually high amylolytic activity in relation to the cyclization activity.

[ 9 ]. CGTase, was selected from B. cereus YUPP-10 by a constructed fos-mid library. We discovered that CGTase has antimicrobial activity and induces resistance.

12 Nov 2019 lans CGTase mutant revealed that this salt bridge is also formed in the mutant. Thus, the activity half-life of this enzyme can be enhanced by

p.514-523 Activity and stability characteristics of an alkaline active cyclodextrin glycosyltransferase (CGTase) enzyme from the alkaliphilic Bacillus agaradhaerens LS-3C strain are reported.

The specific activity was expressed in units of activity by milligram of protein. Protein concentration was estimated according to Hartree (12), using bovine serum albumin as pattern. 2017-10-10 · The β-CGTase activity in the transformant reached 3885.1UmL-1, which is the highest value reported so far, at 28°C, 6% inoculum ratio, and 1.5% methanol addition following 24h of incubation. The recombinant CGTase showed high specific activity at 80°C without any γ-CGTase activity, and had good stability in a wide pH and temperature range. CGTase which showed high specific activity at 80°C without any γ-CGTase activity (Zhang et al.
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yeast extract, peptone and starch showed highest impact on the CGTase production.

When required, different salts such as NaCl, KCl, CaCl2 and NH4Cl were introduced in the reaction mixture in 10-70 mM final The extracellular β-CGTase activity of CGTd4 reached 571.2 U/mL after 57.5 h of fermentation (Fig. 6 a). This result, which was 18.7-fold greater than the activity obtained during shake-flask cultivation, was verified by SDS-PAGE analysis (Fig. 6 b).
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At this point the total soluble γ-CGTase activity had reached 5.51 U·mL − 1. In addition, the ratio of extracellular γ-CGTase activity to total γ-CGTase activity decreased from 71.7 to 55.0% when the concentration of β-cyclodextrin increased from 0 to 10 mM.

689 av J Wouters · 2005 · Citerat av 1 — cgt, CGTase, Differential display, Symbiosis, Nostoc, Gunnera and Gunnera sp. mucilage could not induce nitrogenase activity in darkness. Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; CGTase; cyclodextrin glycosyltransferase; alkyl glycoside; enzyme stabliity; 2 344 4 a- Bacillus megaterium B. macerans CGTase 22 C-2, C-3, C-4 5) A. B., Lapa, A. J., Pharmacological evaluation of the anti-inflammatory activity of a 24 cost | company | product | cost management | activity | sul fab | cgtase | fed-batch cultivation | biolector | dera | autodisplay | enbase. As suggested by activity analysis, expression levels might have been increased in mutated compared to wild-type CGTases in the strains containing the RNaseE product | cost management | activity | sul | agribusiness | environmental cost fab | cgtase | fed-batch cultivation | biolector | dera | autodisplay | enbase 844 C-niveau - KVUC Bild. EP2316929B1 - Maltogenic alpha-amylase variants - Google Patents. Effect of storage temperature on -amylase activity from .